The lipid fraction of annatto (Bixa orellana L.) seeds was extracted with a Soxhlet apparatus with n-hexane and isolated by thin-layer chromatography. The fatty-soluble antioxidant fraction contained only tocotrienols, mainly delta-tocotrienol, but no tocopherols. The presence of tocotrienols was confirmed by gas chromatography-mass spectrometry. The quantities of delta-tocotrienol were 140-147 mg/100 g dry seeds and 5.2-5.5% wt/wt of lipid extract, determined by gas chromatography and high-performance liquid chromatography, respectively. Currently no vegetable species seems to contain comparable concentrations of delta-tocotrienol.
Identification and estimation of tocotrienols in the annatto lipidic fraction using gas chromatography-mass spectrometry / Frega, Natale Giuseppe; Mozzon, Massimo; Bocci, F.. - In: JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY. - ISSN 0003-021X. - ELETTRONICO. - 75:(1998), pp. 1723-1727. [10.1007/s11746-998-0323-1]
Identification and estimation of tocotrienols in the annatto lipidic fraction using gas chromatography-mass spectrometry
FREGA, Natale Giuseppe
;MOZZON, MassimoInvestigation
;
1998-01-01
Abstract
The lipid fraction of annatto (Bixa orellana L.) seeds was extracted with a Soxhlet apparatus with n-hexane and isolated by thin-layer chromatography. The fatty-soluble antioxidant fraction contained only tocotrienols, mainly delta-tocotrienol, but no tocopherols. The presence of tocotrienols was confirmed by gas chromatography-mass spectrometry. The quantities of delta-tocotrienol were 140-147 mg/100 g dry seeds and 5.2-5.5% wt/wt of lipid extract, determined by gas chromatography and high-performance liquid chromatography, respectively. Currently no vegetable species seems to contain comparable concentrations of delta-tocotrienol.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
