BACKGROUND: Breast-fed infants, unlike bottle-fed babies, have a microbic intestinal flora characterised by a marked predominance of bifidobacteria and lactic acid bacteria. This is essentially due to the prebiotic effect of oligosaccharides in human milk. Recently, oligosaccharides with a prebiotic effect have been added to formulas. Aim. To characterise the mixture of oligosaccharides contained in these new formulas. MATERIALS AND METHODS: The characterisation of oligosaccharides was performed using thin layer chromatography as well as high performance anion exchange chromatography. RESULTS: The mixture of oligosaccharides used in the formulas analysed was made up of oligosaccharides with low molecular weight (transgalactosylated oligosaccharides) and polysaccharides with high molecular weight (inulin). CONCLUSION: With the methods employed, it was possible to characterise the mixture of oligosaccharides used as prebiotics in the formulas now available on the market.

Prebiotics in infant formulas: biochemical characterization by thyn layer chromatography and high performance anion exchange chromatography / Coppa, Gv; Bruni, S; Zampini, Lucia; Galeazzi, Tiziana; Gabrielli, Orazio. - In: DIGESTIVE AND LIVER DISEASE. - ISSN 1590-8658. - 34(2):(2002), pp. S124-S128.

Prebiotics in infant formulas: biochemical characterization by thyn layer chromatography and high performance anion exchange chromatography

ZAMPINI, LUCIA;GALEAZZI, Tiziana;GABRIELLI, ORAZIO
2002-01-01

Abstract

BACKGROUND: Breast-fed infants, unlike bottle-fed babies, have a microbic intestinal flora characterised by a marked predominance of bifidobacteria and lactic acid bacteria. This is essentially due to the prebiotic effect of oligosaccharides in human milk. Recently, oligosaccharides with a prebiotic effect have been added to formulas. Aim. To characterise the mixture of oligosaccharides contained in these new formulas. MATERIALS AND METHODS: The characterisation of oligosaccharides was performed using thin layer chromatography as well as high performance anion exchange chromatography. RESULTS: The mixture of oligosaccharides used in the formulas analysed was made up of oligosaccharides with low molecular weight (transgalactosylated oligosaccharides) and polysaccharides with high molecular weight (inulin). CONCLUSION: With the methods employed, it was possible to characterise the mixture of oligosaccharides used as prebiotics in the formulas now available on the market.
2002
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11566/39017
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