A classic solvent extraction and a supercritical CO2 extraction (SFE) method for the determination of free cholesterol in dried egg yolk were compared. After lipid extraction, a solid-phase extraction (SPE) was performed to eliminate the non-polar lipids and to purify cholesterol. Free cholesterol was then determined by capillary gas chromatography. The supercritical extractor enabled on line SPE after SFE, resulting in a shorter extraction time compared with that of the classic method. Improved repeatability and a smaller amount of solvent required are the main advantages of the method proposed here compared with the classic solvent extraction method.
Determination of Free Cholesterol from dried Egg Yolk by on-line Coupling of Supercritical Fluid Extraction with Solid Phase Extraction / Boselli, Emanuele; M. F. CABONI AND G., Lercker. - In: ZEITSCHRIFT FUER LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG. A-FOOD RESEARCH AND TECHNOLOGY. - ISSN 1431-4630. - 205:(1997), pp. 356-359. [10.1007/s002170050180]
Determination of Free Cholesterol from dried Egg Yolk by on-line Coupling of Supercritical Fluid Extraction with Solid Phase Extraction
BOSELLI, EMANUELE;
1997-01-01
Abstract
A classic solvent extraction and a supercritical CO2 extraction (SFE) method for the determination of free cholesterol in dried egg yolk were compared. After lipid extraction, a solid-phase extraction (SPE) was performed to eliminate the non-polar lipids and to purify cholesterol. Free cholesterol was then determined by capillary gas chromatography. The supercritical extractor enabled on line SPE after SFE, resulting in a shorter extraction time compared with that of the classic method. Improved repeatability and a smaller amount of solvent required are the main advantages of the method proposed here compared with the classic solvent extraction method.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
