A classic solvent extraction and a supercritical CO2 extraction (SFE) method for the determination of free cholesterol in dried egg yolk were compared. After lipid extraction, a solid-phase extraction (SPE) was performed to eliminate the non-polar lipids and to purify cholesterol. Free cholesterol was then determined by capillary gas chromatography. The supercritical extractor enabled on line SPE after SFE, resulting in a shorter extraction time compared with that of the classic method. Improved repeatability and a smaller amount of solvent required are the main advantages of the method proposed here compared with the classic solvent extraction method.

Determination of Free Cholesterol from dried Egg Yolk by on-line Coupling of Supercritical Fluid Extraction with Solid Phase Extraction

BOSELLI, EMANUELE;
1997

Abstract

A classic solvent extraction and a supercritical CO2 extraction (SFE) method for the determination of free cholesterol in dried egg yolk were compared. After lipid extraction, a solid-phase extraction (SPE) was performed to eliminate the non-polar lipids and to purify cholesterol. Free cholesterol was then determined by capillary gas chromatography. The supercritical extractor enabled on line SPE after SFE, resulting in a shorter extraction time compared with that of the classic method. Improved repeatability and a smaller amount of solvent required are the main advantages of the method proposed here compared with the classic solvent extraction method.
File in questo prodotto:
File Dimensione Formato  
ZLUF1997chol.pdf

non disponibili

Tipologia: Documento in Post-print
Licenza: NON PUBBLICO-Accesso privato/ristretto
Dimensione 127.79 kB
Formato Adobe PDF
127.79 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11566/35876
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact