Phospholipids (PL) were successfully extracted from dried egg yolk by using neat supercritical carbon dioxide (SC-CO2) on a microscale. The spray-dried egg yolk powder was extracted with SC-CO2 at a density of 1.06 g/ml (517 bar, 40 degreesC), which was the maximum of density achievable with the apparatus. The extraction yield obtained by SC-CO2 (67 g of extract per 100 g of sample) can be compared with the conventional Bligh and Dyer extraction method (63 g of extract per 100 g of sample), which involves a chloroform/methanol/water mixture as an extraction solvent. The solvent extract contained 29% of PL, while the neat SC-CO2 extract consisted of 26% PL. HPLC analysis coupled with an evaporative light scattering detector (ELSD) showed no significant selectivity in the SC-CO2 extraction of the PL components. The extraction with neat SC-CO2 appears to be a valid alter-native for the extraction of PL for analytical purpose. (C) 2000 Elsevier Science B.V. All rights reserved.
Supercritical carbon dioxide extraction of phospholipids from dried egg yolk without organic modifier
BOSELLI, EMANUELE;
2000-01-01
Abstract
Phospholipids (PL) were successfully extracted from dried egg yolk by using neat supercritical carbon dioxide (SC-CO2) on a microscale. The spray-dried egg yolk powder was extracted with SC-CO2 at a density of 1.06 g/ml (517 bar, 40 degreesC), which was the maximum of density achievable with the apparatus. The extraction yield obtained by SC-CO2 (67 g of extract per 100 g of sample) can be compared with the conventional Bligh and Dyer extraction method (63 g of extract per 100 g of sample), which involves a chloroform/methanol/water mixture as an extraction solvent. The solvent extract contained 29% of PL, while the neat SC-CO2 extract consisted of 26% PL. HPLC analysis coupled with an evaporative light scattering detector (ELSD) showed no significant selectivity in the SC-CO2 extraction of the PL components. The extraction with neat SC-CO2 appears to be a valid alter-native for the extraction of PL for analytical purpose. (C) 2000 Elsevier Science B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
