Nutrient quality, with emphasis on lipid and fatty acid composition of broodstock diet, is one of the major dietary factors influencing fish oocytes maturation, reproductive success and offspring quality/survival. The Black Soldier Fly (BFS) (Hermetia illucens) is a valuable insect species characterized by a balanced essential amino acid profile comparable to fishmeal and has been demonstrated to be an eco-friendly and low-cost protein source in fish diets. However, as regards lipids, BFS is usually characterized by a poor content in polyunsaturated fatty acids which are essential for fish during critical life-cycle stages including oocyte maturation and reproduction. In the present study, full-fat enriched BSF prepupae meal was obtained from BSF larvae reared on coffee by-product (coffee silverskin) enriched with 10% of Schizochytrium sp. Successively, five experimental diets containing different full-fat enriched BSF prepupae meal inclusion levels (corresponding to the 0, 25, 50, 75 and 100% fish meal replacement) were tested during a six-month feeding trial performed on zebrafish (Danio rerio) to investigate, for the first time, the physiological effects on female fish growth, stress response, lipid metabolism and reproductive performances. A multidisciplinary approach including biometric, gas-chromatographic, histological and molecular analyses was used during the present study to have a comprehensive overview of fish responses to the test diets. Results highlighted that up to 50% of fish meal replacement with enriched BSF meal did not impair zebrafish physiological responses while higher replacement levels (75 and 100%) negatively affected fish stress response, oocytes maturation stages, spawning and hatching success.

Effects of black soldier fly (Hermetia illucens) enriched with Schizochytrium sp. on zebrafish (Danio rerio) reproductive performances / Chemello, G.; Zarantoniello, M.; Randazzo, B.; Gioacchini, G.; Truzzi, C.; Cardinaletti, G.; Riolo, P.; Olivotto, I.. - In: AQUACULTURE. - ISSN 0044-8486. - ELETTRONICO. - 550:(2022), p. 737853. [10.1016/j.aquaculture.2021.737853]

Effects of black soldier fly (Hermetia illucens) enriched with Schizochytrium sp. on zebrafish (Danio rerio) reproductive performances

Chemello G.;Zarantoniello M.;Randazzo B.;Gioacchini G.;Truzzi C.;Riolo P.;Olivotto I.
2022-01-01

Abstract

Nutrient quality, with emphasis on lipid and fatty acid composition of broodstock diet, is one of the major dietary factors influencing fish oocytes maturation, reproductive success and offspring quality/survival. The Black Soldier Fly (BFS) (Hermetia illucens) is a valuable insect species characterized by a balanced essential amino acid profile comparable to fishmeal and has been demonstrated to be an eco-friendly and low-cost protein source in fish diets. However, as regards lipids, BFS is usually characterized by a poor content in polyunsaturated fatty acids which are essential for fish during critical life-cycle stages including oocyte maturation and reproduction. In the present study, full-fat enriched BSF prepupae meal was obtained from BSF larvae reared on coffee by-product (coffee silverskin) enriched with 10% of Schizochytrium sp. Successively, five experimental diets containing different full-fat enriched BSF prepupae meal inclusion levels (corresponding to the 0, 25, 50, 75 and 100% fish meal replacement) were tested during a six-month feeding trial performed on zebrafish (Danio rerio) to investigate, for the first time, the physiological effects on female fish growth, stress response, lipid metabolism and reproductive performances. A multidisciplinary approach including biometric, gas-chromatographic, histological and molecular analyses was used during the present study to have a comprehensive overview of fish responses to the test diets. Results highlighted that up to 50% of fish meal replacement with enriched BSF meal did not impair zebrafish physiological responses while higher replacement levels (75 and 100%) negatively affected fish stress response, oocytes maturation stages, spawning and hatching success.
2022
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11566/294382
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 14
  • ???jsp.display-item.citation.isi??? 12
social impact