OBJECTIVES: An aging-suppressor gene, klotho, is a candidate factor for vascular disease because its deficiency leads to impaired endothelium-dependent vasodilation and impaired angiogenesis. The aim was to verify a possible relation among the expression of the klotho gene, single nucleotide polymorphisms (SNP) in the promoter region, and placenta aging. METHODS: Placentas were collected from normal pregnancies (n=34) and pregnancies complicated by Preeclampsia (n=34), matched for gestational age. Klotho mRNA and protein were determined using Real-Time PCR and Western blot, respectively. SNPs (i.e.: -744delA, and -395A/G) were investigated using allele-specific PCR. Expression of pluripotency markers (i.e.: Nanog, and Oct-4) and telomere length measurement were assessed using Real-Time PCR. RESULTS: Real-Time PCR analyses demonstrated a significant down-regulation of Klotho ( 83%; p=0.005) in patients with Preeclampsia versus Controls. Results of Western Blot agreed with Real-Time PCR ones. Polymorphism analysis results suggest that -744delA allele is associated with 3-fold increased risk for preeclampsia. Real-Time PCR investigation revealed a significant down-regulation of pluripotency markers in pathological group. CONCLUSIONS: Klotho expression is decreased in preeclamptic pregnancies. Further data are required to confirm the role of this protein in pathophysiology of preeclampsia and the possible link to long term outcomes.
[310-POS]: Potential role of Klotho protein in the pathophysiology of preeclampsia / Cecati, Monia; Giannubilo, Stefano Raffaele; Corradetti, Alessandra; Emanuelli, Monica; Saccucci, Franca; Tranquilli, Andrea Luigi. - In: PREGNANCY HYPERTENSION. - ISSN 2210-7789. - STAMPA. - 5:(2015), pp. 152-3-153. [10.1016/j.preghy.2014.10.316]
[310-POS]: Potential role of Klotho protein in the pathophysiology of preeclampsia
CECATI, Monia;GIANNUBILO, Stefano Raffaele;CORRADETTI, ALESSANDRA;EMANUELLI, Monica;SACCUCCI, FRANCA;TRANQUILLI, Andrea Luigi
2015-01-01
Abstract
OBJECTIVES: An aging-suppressor gene, klotho, is a candidate factor for vascular disease because its deficiency leads to impaired endothelium-dependent vasodilation and impaired angiogenesis. The aim was to verify a possible relation among the expression of the klotho gene, single nucleotide polymorphisms (SNP) in the promoter region, and placenta aging. METHODS: Placentas were collected from normal pregnancies (n=34) and pregnancies complicated by Preeclampsia (n=34), matched for gestational age. Klotho mRNA and protein were determined using Real-Time PCR and Western blot, respectively. SNPs (i.e.: -744delA, and -395A/G) were investigated using allele-specific PCR. Expression of pluripotency markers (i.e.: Nanog, and Oct-4) and telomere length measurement were assessed using Real-Time PCR. RESULTS: Real-Time PCR analyses demonstrated a significant down-regulation of Klotho ( 83%; p=0.005) in patients with Preeclampsia versus Controls. Results of Western Blot agreed with Real-Time PCR ones. Polymorphism analysis results suggest that -744delA allele is associated with 3-fold increased risk for preeclampsia. Real-Time PCR investigation revealed a significant down-regulation of pluripotency markers in pathological group. CONCLUSIONS: Klotho expression is decreased in preeclamptic pregnancies. Further data are required to confirm the role of this protein in pathophysiology of preeclampsia and the possible link to long term outcomes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.