Two particle-associated antigens were isolated by immunoaffinity chromatography from hepatitis non-A, non-B chimpanzee or patient sera. The immunoadsorbent was prepared by coupling the Affi-Prep 10 matrix with immunoglobulins of a chimpanzee immunized with inactivated viral lysate. An antigen estimated to be 33•5 kilodaltons was isolated from acute phase sera of a hepatitis non-A, non-B infected chimpanzee. Western blot analyses showed that this antigen is immunoreactive using a chronic patient serum as the source of antibodies. The antigen was not found in the preinoculation serum. In addition to the 33•5 kilodalton protein, a second antigen estimated to be 56 kilodaltons was isolated from the serum of an acute hepatitis non-A, non-B patient.

The use of immunoaffinity chromatography for the isolation of antigens in hepatitis non-A non-B sera / Cacciamani, Tiziana; Belinda, Seto. - In: SERODIAGNOSIS AND IMMUNOTHERAPY IN INFECTIOUS DISEASE. - ISSN 0888-0786. - ELETTRONICO. - 3:6(1989), pp. 383-388. [10.1016/0888-0786(89)90056-5]

The use of immunoaffinity chromatography for the isolation of antigens in hepatitis non-A non-B sera

CACCIAMANI, Tiziana;
1989-01-01

Abstract

Two particle-associated antigens were isolated by immunoaffinity chromatography from hepatitis non-A, non-B chimpanzee or patient sera. The immunoadsorbent was prepared by coupling the Affi-Prep 10 matrix with immunoglobulins of a chimpanzee immunized with inactivated viral lysate. An antigen estimated to be 33•5 kilodaltons was isolated from acute phase sera of a hepatitis non-A, non-B infected chimpanzee. Western blot analyses showed that this antigen is immunoreactive using a chronic patient serum as the source of antibodies. The antigen was not found in the preinoculation serum. In addition to the 33•5 kilodalton protein, a second antigen estimated to be 56 kilodaltons was isolated from the serum of an acute hepatitis non-A, non-B patient.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11566/87698
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