The malignant mesothelioma (MM) is often complicated by thromboembolic episodes, with thrombomodulin (TM) playing a role in the anti-coagulant process. We analyzed TM expression in biopsies of MM patients and in normal mesothelial tissue. The role of DNA methylation-associated gene silencing in TM expression was investigated. A correlation between low TM expression and high level of TM promoter methylation was found in MM biopsies. Low expression of TM was restored in MM cells by their treatment with 5-aza-2’-deoxycytidine while the epigenetic agent did not affect TM expression in Met-5A cells. Methylation of the TM promoter is responsible for silencing of TM expression in MM tissue.
Expression of thrombomodulin in malignant pleural mesothelioma / Amati, Monica; Nocchi, Linda; Tomasetti, Marco; Santarelli, Lory; Saccucci, Franca. - In: GIORNALE ITALIANO DI MEDICINA DEL LAVORO ED ERGONOMIA. - ISSN 1592-7830. - STAMPA. - 33:(2011), pp. 99-102.
Expression of thrombomodulin in malignant pleural mesothelioma
AMATI, Monica;NOCCHI, LINDA;TOMASETTI, Marco;SANTARELLI, Lory;SACCUCCI, FRANCA
2011-01-01
Abstract
The malignant mesothelioma (MM) is often complicated by thromboembolic episodes, with thrombomodulin (TM) playing a role in the anti-coagulant process. We analyzed TM expression in biopsies of MM patients and in normal mesothelial tissue. The role of DNA methylation-associated gene silencing in TM expression was investigated. A correlation between low TM expression and high level of TM promoter methylation was found in MM biopsies. Low expression of TM was restored in MM cells by their treatment with 5-aza-2’-deoxycytidine while the epigenetic agent did not affect TM expression in Met-5A cells. Methylation of the TM promoter is responsible for silencing of TM expression in MM tissue.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
