OBJECTIVE: The objective of this study was to determine whether cultured trophoblast cells shared the same morphological and biological properties observed in trophoblast, in vivo. STUDY DESIGN: Trophoblast cells from human term placenta were cultured, morphologically, biochemically and immunochemically monitored for as long as 30 days. RESULTS: Single cells progressively aggregated and fused into a syncytio, the Ca2+ and the Ca(2+)-ATPase activity drooped, and the 72 kDa collagenase (MMP-2) was consistently expressed. CONCLUSIONS: Term placenta trophoblast cultures can be viewed and used as a model system mimicking morphological and biochemical events of placenta biology and differentiation.
Cultured human trophoblast cells reproduce the initial events of placental biology / S., Garbisa; M., Onisto; Mazzanti, Laura; Tranquilli, Andrea Luigi; Pugnaloni, Armanda; G., Biagini; N., Cester; C., Romanini. - In: CLINICAL AND EXPERIMENTAL OBSTETRICS & GYNECOLOGY. - ISSN 0390-6663. - STAMPA. - 20:4(1993), pp. 207-215.
Cultured human trophoblast cells reproduce the initial events of placental biology.
MAZZANTI, LAURA;TRANQUILLI, Andrea Luigi;PUGNALONI, Armanda;
1993-01-01
Abstract
OBJECTIVE: The objective of this study was to determine whether cultured trophoblast cells shared the same morphological and biological properties observed in trophoblast, in vivo. STUDY DESIGN: Trophoblast cells from human term placenta were cultured, morphologically, biochemically and immunochemically monitored for as long as 30 days. RESULTS: Single cells progressively aggregated and fused into a syncytio, the Ca2+ and the Ca(2+)-ATPase activity drooped, and the 72 kDa collagenase (MMP-2) was consistently expressed. CONCLUSIONS: Term placenta trophoblast cultures can be viewed and used as a model system mimicking morphological and biochemical events of placenta biology and differentiation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
