The in vitro susceptibilities of 90 clinical isolates of gram-positive and gram-negative aerobic bacteria to six cationic peptides, buforin II, cecropin P1, indolicidin, magainin II, nisin, and ranalexin, were evaluated by two broth microdilution methods. The first method was performed according to the procedures outlined by the National Committee for Clinical Laboratory Standards for bacteria that grow aerobically, while the second was performed according to the procedures recently proposed by the R. E. W. Hancock laboratory for testing antimicrobial peptides. Overall, the first method produced MICs two- and fourfold higher than the second method.
In vitro susceptibility tests for cationic peptides: comparison of broth microdilution methods for bacteria that grow aerobically / Giacometti, Andrea; Cirioni, Oscar; Barchiesi, Francesco; Del Prete, Ms; Fortuna, M; Caselli, F; Scalise, Giorgio. - In: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. - ISSN 0066-4804. - STAMPA. - 44:6(2000), pp. 1694-1696.
In vitro susceptibility tests for cationic peptides: comparison of broth microdilution methods for bacteria that grow aerobically.
GIACOMETTI, Andrea;CIRIONI, OSCAR;BARCHIESI, FRANCESCO;SCALISE, Giorgio
2000-01-01
Abstract
The in vitro susceptibilities of 90 clinical isolates of gram-positive and gram-negative aerobic bacteria to six cationic peptides, buforin II, cecropin P1, indolicidin, magainin II, nisin, and ranalexin, were evaluated by two broth microdilution methods. The first method was performed according to the procedures outlined by the National Committee for Clinical Laboratory Standards for bacteria that grow aerobically, while the second was performed according to the procedures recently proposed by the R. E. W. Hancock laboratory for testing antimicrobial peptides. Overall, the first method produced MICs two- and fourfold higher than the second method.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.