OBJECTIVES: Aim of this study was to evaluate the accuracy and precision of the detection of individual miRNA as clinical biomarkers in the serum. DESIGN AND METHODS: miRNA-126 was quantified in serum using endogenous and exogenous controls for normalization and the accuracy and precision of the method evaluated. The diagnostic value of serum miRNA-126 was evaluated in malignant mesothelioma (MM) and non-small-cell lung cancer (NSCLC) patients using both relative and absolute qRT-PCR methods. RESULTS: The use of endogenous invariant and exogenous synthetic controls as well sample dilution markedly improves the accuracy and precision of the assay. The inter- and intra-assay analyses revealed that relative qRT-PCR is a more reliable method. Circulating miR-126 detected in the serum by relative qRT-PCRs was found low-expressed in both malignancies, significantly differentiated MM patients from healthy controls and NSCLC from MM, but do not discriminate NSCLC patients from control subjects. Kaplan-Meier analysis revealed that low level of circulating miR-126 in MM patients was strongly associated with worse prognosis. CONCLUSIONS: We propose that this approach can be adopted for accurate analysis of other suitable circulating miRNA markers of different types of cancer.
Clinical significance of circulating miR-126 quantification in malignant mesothelioma patients / Tomasetti, Marco; Staffolani, Sara; Nocchi, Linda; Neuzil, J; Strafella, Elisabetta; Manzella, Nicola; Mariotti, Laura; Bracci, Massimo; Valentino, Matteo; Amati, Monica; Santarelli, Lory. - In: CLINICAL BIOCHEMISTRY. - ISSN 0009-9120. - 45:7-8(2012), pp. 575-581. [10.1016/j.clinbiochem.2012.02.009]
Clinical significance of circulating miR-126 quantification in malignant mesothelioma patients
TOMASETTI, Marco;STAFFOLANI, SARA;NOCCHI, LINDA;STRAFELLA, ELISABETTA;MANZELLA, NICOLA;MARIOTTI, LAURA;BRACCI, MASSIMO;VALENTINO, Matteo;AMATI, Monica;SANTARELLI, Lory
2012-01-01
Abstract
OBJECTIVES: Aim of this study was to evaluate the accuracy and precision of the detection of individual miRNA as clinical biomarkers in the serum. DESIGN AND METHODS: miRNA-126 was quantified in serum using endogenous and exogenous controls for normalization and the accuracy and precision of the method evaluated. The diagnostic value of serum miRNA-126 was evaluated in malignant mesothelioma (MM) and non-small-cell lung cancer (NSCLC) patients using both relative and absolute qRT-PCR methods. RESULTS: The use of endogenous invariant and exogenous synthetic controls as well sample dilution markedly improves the accuracy and precision of the assay. The inter- and intra-assay analyses revealed that relative qRT-PCR is a more reliable method. Circulating miR-126 detected in the serum by relative qRT-PCRs was found low-expressed in both malignancies, significantly differentiated MM patients from healthy controls and NSCLC from MM, but do not discriminate NSCLC patients from control subjects. Kaplan-Meier analysis revealed that low level of circulating miR-126 in MM patients was strongly associated with worse prognosis. CONCLUSIONS: We propose that this approach can be adopted for accurate analysis of other suitable circulating miRNA markers of different types of cancer.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.