Grazing-incidence small-angle X-ray scattering (GISAXS) has been used to study proteins embedded in thin polymer films obtained by a new cold, atmospheric-pressure plasma technique. In order to test the efficiency of the technology, four samples of alkaline phosphatase incorporated in organic polymer coatings in different plasma conditions have been investigated. Data have been analysed in the framework of the distorted-wave Born approximation (DWBA), by using a new method for the simultaneous fitting of the two-dimensional diffuse scattering from each sample. As a result, protein film concentration and aggregation state as well as a set of parameters describing the polymer coatings have been obtained.
Grazing-incidence small-angle x-ray scattering from alkaline phosphatase immobilized in atmospheric plasmapolymer coatings / Ortore, Maria Grazia; R., Sinibaldi; P., Heyse; S., Paulussen; S., Bernstorff; B., Sels; Mariani, Paolo; Rustichelli, Franco; Spinozzi, Francesco. - In: APPLIED SURFACE SCIENCE. - ISSN 0169-4332. - STAMPA. - 254:(2008), pp. 5557-5563. [10.1016/j.apsusc.2008.02.118]
Grazing-incidence small-angle x-ray scattering from alkaline phosphatase immobilized in atmospheric plasmapolymer coatings
ORTORE, Maria Grazia;MARIANI, Paolo;RUSTICHELLI, Franco;SPINOZZI, Francesco
2008-01-01
Abstract
Grazing-incidence small-angle X-ray scattering (GISAXS) has been used to study proteins embedded in thin polymer films obtained by a new cold, atmospheric-pressure plasma technique. In order to test the efficiency of the technology, four samples of alkaline phosphatase incorporated in organic polymer coatings in different plasma conditions have been investigated. Data have been analysed in the framework of the distorted-wave Born approximation (DWBA), by using a new method for the simultaneous fitting of the two-dimensional diffuse scattering from each sample. As a result, protein film concentration and aggregation state as well as a set of parameters describing the polymer coatings have been obtained.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
