When mixing aqueous solutions of DNA and dioloeyl-phosphatidylcholine multilamellar liposomes in the presence of Mn 2+ ions, a multilamellar liquid-crystalline phase is formed in which DNA monolayers are comprised between opposing lipid bilayers. Here, we report on a new experimental procedure resulting in the formation of solid-supported DOPC-DNA-Mn 2+ complexes in the biologically relevant excess water condition. The supramolecular structure of solid-supported complexes, characterized by means of Energy Dispersive X-ray Diffraction, exhibits very similar structural properties with respect to the solution structure of the complexes as revealed by synchrotron Small Angle X-ray Scattering measurements.
Structure of solid-supported lipid-DNA-metal complexes investigated by energy dispersive X-ray diffraction / Caracciolo, G; Sadum, C; Caminiti, R; Pisani, Michela; Bruni, Paolo; Francescangeli, Oriano. - In: CHEMICAL PHYSICS LETTERS. - ISSN 0009-2614. - 397 (1-3):(2004), pp. 138-143. [10.1016/j.cplett.2004.08.102]
Structure of solid-supported lipid-DNA-metal complexes investigated by energy dispersive X-ray diffraction
PISANI, MICHELA;BRUNI, PAOLO;FRANCESCANGELI, ORIANO
2004-01-01
Abstract
When mixing aqueous solutions of DNA and dioloeyl-phosphatidylcholine multilamellar liposomes in the presence of Mn 2+ ions, a multilamellar liquid-crystalline phase is formed in which DNA monolayers are comprised between opposing lipid bilayers. Here, we report on a new experimental procedure resulting in the formation of solid-supported DOPC-DNA-Mn 2+ complexes in the biologically relevant excess water condition. The supramolecular structure of solid-supported complexes, characterized by means of Energy Dispersive X-ray Diffraction, exhibits very similar structural properties with respect to the solution structure of the complexes as revealed by synchrotron Small Angle X-ray Scattering measurements.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
