The new lipid 1,2-O-dioleyl-3-O-{2-[(12-crown-4)-methoxy]-ethyl}-sn-glycerol, 12C4L, has been synthesized. This molecule can coordinate different cations that should make the corresponding liposome a good candidate as vector of genetic material for possible applications in gene therapy. An important feature of the molecule is the possibility to modulate the net surface charge of their complexes with metal cations, which is important to provide efficient DNA transfections. The molecule and its complexes with some metal cations (Mg2+, Ca2+, Mn2+) have been characterized by FT-IR spectroscopy and band attributions confirmed by Density Functional Theory calculations. The net surface charge has been determined by Z potential determinations.
12-Crown-4-based amphipatic lipid and corresponding metal cation complexes for gene therapy applications: FT-IR characterization and surface charge determination / Bruni, P; Fino, V; Pisani, Michela; Tosi, Giorgio; Stipa, Pierluigi; Ferraris, P; Francescangeli, Oriano. - In: JOURNAL OF MOLECULAR STRUCTURE. - ISSN 0022-2860. - 919:(2009), pp. 328-333. [10.1016/j.molstruc.2008.09.030]
12-Crown-4-based amphipatic lipid and corresponding metal cation complexes for gene therapy applications: FT-IR characterization and surface charge determination
PISANI, MICHELA;TOSI, Giorgio;STIPA, Pierluigi;FRANCESCANGELI, ORIANO
2009-01-01
Abstract
The new lipid 1,2-O-dioleyl-3-O-{2-[(12-crown-4)-methoxy]-ethyl}-sn-glycerol, 12C4L, has been synthesized. This molecule can coordinate different cations that should make the corresponding liposome a good candidate as vector of genetic material for possible applications in gene therapy. An important feature of the molecule is the possibility to modulate the net surface charge of their complexes with metal cations, which is important to provide efficient DNA transfections. The molecule and its complexes with some metal cations (Mg2+, Ca2+, Mn2+) have been characterized by FT-IR spectroscopy and band attributions confirmed by Density Functional Theory calculations. The net surface charge has been determined by Z potential determinations.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.