These findings contribute to conclusively clarifying why, among S. pyogenes isolates with the M phenotype, the tetracycline- resistant ones are, as a rule, SmaI typeable, while the tetracycline-susceptible ones are not. Previous studies have shown that, while the two related genetic elements (Tn1207.3 and 10394.4) typical of tetracycline-susceptible isolates are inserted into the same prophage, the tet(O)-mef(A) element typical of tetracycline-resistant isolates is inserted into a different prophage (6). The present results show that the R-M cassette encoding, in Tn1207.3-10394.4, the methyltransferase responsible for DNA resistance to cleavage by SmaI is absent in the tet(O)-mef(A) element where, together with an adjacent ORF, it is replaced by three new ORFs flanked, both upstream and downstream, by homologous regions.

SmaI typeability and tetracycline susceptibility/resistance in Streptococcus pyogenes isolates with efflux-mediated erythromycin resistance / Bacciaglia, Alessandro; Brenciani, Andrea; Varaldo, Pietro; Giovanetti, Eleonora. - In: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. - ISSN 0066-4804. - 51:(2007), pp. 3042-3043. [10.1128/AAC.00249-07]

SmaI typeability and tetracycline susceptibility/resistance in Streptococcus pyogenes isolates with efflux-mediated erythromycin resistance.

BACCIAGLIA, ALESSANDRO;BRENCIANI, Andrea;VARALDO, Pietro;GIOVANETTI, Eleonora
2007-01-01

Abstract

These findings contribute to conclusively clarifying why, among S. pyogenes isolates with the M phenotype, the tetracycline- resistant ones are, as a rule, SmaI typeable, while the tetracycline-susceptible ones are not. Previous studies have shown that, while the two related genetic elements (Tn1207.3 and 10394.4) typical of tetracycline-susceptible isolates are inserted into the same prophage, the tet(O)-mef(A) element typical of tetracycline-resistant isolates is inserted into a different prophage (6). The present results show that the R-M cassette encoding, in Tn1207.3-10394.4, the methyltransferase responsible for DNA resistance to cleavage by SmaI is absent in the tet(O)-mef(A) element where, together with an adjacent ORF, it is replaced by three new ORFs flanked, both upstream and downstream, by homologous regions.
2007
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11566/50844
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 11
social impact