Bone marrow is a useful cell source for skeletal tissue engineering approaches. In vitro differentiation of marrow mesenchymal stem cells (MSCs) to chondrocytes or osteoblasts can be induced by the addition of specific growth factors to the medium. The present study evaluated the behaviour of human MSCs cultured on various scaffolds to determine whether their differentiation can be induced by cell-matrix interactions.MSCs from bone marrow collected from the acetabulum during hip arthroplasty procedures were isolated by cell sorting, expanded and characterised by a flow cytometry system. Cells were grown on three different scaffolds (type I collagen, type I + II collagen and type I collagen + hydroxyapatite membranes) and analysed by histochemistry, immunohistochemistry and spectrophotometry (cell proliferation, alkaline phosphatase activity) at 15 and 30 days.Widely variable cell adhesion and proliferation was observed on the three scaffolds. MSCs grown on type I + II collagen differentiated to cells expressing chondrocyte markers, while those grown on type I collagen + hydroxyapatite differentiated into osteoblast-like cells.The study highlighted that human MSCs grown on different scaffold matrices may display different behaviours in terms of cell proliferation and phenotype expression without growth factor supplementation. (C) 2008 European Journal of Histochemistry
Adult mesenchymal stem cells for bone and cartilage engineering: effect of scaffold materials / Gigante, ANTONIO POMPILIO; Manzotti, Sandra; Bevilacqua, C.; Orciani, Monia; DI PRIMIO, Roberto; MATTIOLI BELMONTE CIMA, Monica. - In: EUROPEAN JOURNAL OF HISTOCHEMISTRY. - ISSN 1121-760X. - 52:3(2008), pp. 169-174.
Adult mesenchymal stem cells for bone and cartilage engineering: effect of scaffold materials
GIGANTE, ANTONIO POMPILIO;MANZOTTI, SANDRA;ORCIANI, Monia;DI PRIMIO, Roberto;MATTIOLI BELMONTE CIMA, Monica
2008-01-01
Abstract
Bone marrow is a useful cell source for skeletal tissue engineering approaches. In vitro differentiation of marrow mesenchymal stem cells (MSCs) to chondrocytes or osteoblasts can be induced by the addition of specific growth factors to the medium. The present study evaluated the behaviour of human MSCs cultured on various scaffolds to determine whether their differentiation can be induced by cell-matrix interactions.MSCs from bone marrow collected from the acetabulum during hip arthroplasty procedures were isolated by cell sorting, expanded and characterised by a flow cytometry system. Cells were grown on three different scaffolds (type I collagen, type I + II collagen and type I collagen + hydroxyapatite membranes) and analysed by histochemistry, immunohistochemistry and spectrophotometry (cell proliferation, alkaline phosphatase activity) at 15 and 30 days.Widely variable cell adhesion and proliferation was observed on the three scaffolds. MSCs grown on type I + II collagen differentiated to cells expressing chondrocyte markers, while those grown on type I collagen + hydroxyapatite differentiated into osteoblast-like cells.The study highlighted that human MSCs grown on different scaffold matrices may display different behaviours in terms of cell proliferation and phenotype expression without growth factor supplementation. (C) 2008 European Journal of HistochemistryI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.