A simple ultra high resolution (UHR)-size exclusion HPLC (SEC-HPLC) system with online detection by ICP-MS was set-up to quantify metallothioneins (MT), Zn bound to MT and to other cytosolic protein fractions with defined molecular weight in human peripheral blood mononuclear cells (PBMC). Applications of this method in combination with flow cytometry (FC) offer powerful diagnostic approaches for the evaluation of the zinc status and for quantifying MT in a very small sample amount. © The Royal Society of Chemistry.
Combining UHR-SEC-HPLC-ICP-MS with flow cytometry to quantify metallothioneins and to study zinc homeostasis in human PBMC / Malavolta, M.; Piacenza, F.; Costarelli, L.; Giacconi, R.; Muti, E.; Cipriano, C.; Tesei, S.; Spezia, S.; Mocchegiani, E.. - In: JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. - ISSN 0267-9477. - 22:9(2007), pp. 1193-1198. [10.1039/b704577h]
Combining UHR-SEC-HPLC-ICP-MS with flow cytometry to quantify metallothioneins and to study zinc homeostasis in human PBMC
Malavolta M.
Primo
Conceptualization
;
2007-01-01
Abstract
A simple ultra high resolution (UHR)-size exclusion HPLC (SEC-HPLC) system with online detection by ICP-MS was set-up to quantify metallothioneins (MT), Zn bound to MT and to other cytosolic protein fractions with defined molecular weight in human peripheral blood mononuclear cells (PBMC). Applications of this method in combination with flow cytometry (FC) offer powerful diagnostic approaches for the evaluation of the zinc status and for quantifying MT in a very small sample amount. © The Royal Society of Chemistry.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
