Aim: To evaluate CpG methylation of long interspersed nuclear elements 1 (LINE-1) and human endogenous retrovirus K (HERV-K) retroelements as potential prognostic biomarkers in cutaneous melanoma. Materials methods: Methylation of HERV-K and LINE-1 retroelements was assessed in resected melanoma tissues from 82 patients ranging in age from 14 to 88 years. In addition, nevi from eight patients were included for comparison with nonmalignant melanocytic lesions. Results: Methylation levels were lower in melanomas than in nevi. HERV-K and LINE-1 methylation were decreased in melanoma patients with clinical parameters associated with adverse prognosis, while they were independent of age and gender. Hypomethylation of HERV-K (but not LINE-1) was an independent predictor of reduced disease-free survival. Conclusion: HERV-K hypomethylation can be a potential independent biomarker of melanoma recurrence.
Association of HERV-K and LINE-1 hypomethylation with reduced disease-free survival in melanoma patients / Cardelli, M.; Doorn, R. V.; Larcher, L.; Donato, M. D.; Piacenza, F.; Pierpaoli, E.; Giacconi, R.; Malavolta, M.; Rachakonda, S.; Gruis, N. A.; Molven, A.; Andresen, P. A.; Pjanova, D.; Van Den Oord, J. J.; Provinciali, M.; Nagore, E.; Kumar, R.. - In: EPIGENOMICS. - ISSN 1750-1911. - 12:19(2020), pp. 1689-1706. [10.2217/epi-2020-0127]
Association of HERV-K and LINE-1 hypomethylation with reduced disease-free survival in melanoma patients
Piacenza F.;Pierpaoli E.;Malavolta M.Writing – Review & Editing
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2020-01-01
Abstract
Aim: To evaluate CpG methylation of long interspersed nuclear elements 1 (LINE-1) and human endogenous retrovirus K (HERV-K) retroelements as potential prognostic biomarkers in cutaneous melanoma. Materials methods: Methylation of HERV-K and LINE-1 retroelements was assessed in resected melanoma tissues from 82 patients ranging in age from 14 to 88 years. In addition, nevi from eight patients were included for comparison with nonmalignant melanocytic lesions. Results: Methylation levels were lower in melanomas than in nevi. HERV-K and LINE-1 methylation were decreased in melanoma patients with clinical parameters associated with adverse prognosis, while they were independent of age and gender. Hypomethylation of HERV-K (but not LINE-1) was an independent predictor of reduced disease-free survival. Conclusion: HERV-K hypomethylation can be a potential independent biomarker of melanoma recurrence.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
