We investigated quadruplex formation in aqueous solutions of 2′ -deoxyriboguanosine 5′ -monophosphate, d(pG), which takes place in the absence of the covalent axial backbone. A series of in-solution small angle X-ray scattering experiments on d(pG) have been performed as a function of temperature in the absence of excess salt, at a concentration just above the critical one at which self-assembling occurs. A global fit approach has been used to derive composition and size distribution of the scattering particles as a function of temperature. The obtained results give thermodynamical justification for the observed phase-behavior, indicating that octamer formation is essential for quadruplex elongation. Our investigation shows that d(pG) quadruplexes are very suitable to assess the potential of G-quadruplex formation and to study the self-assembling thermodynamics.

Guanosine Quadruplexes in Solution: A Small-Angle X-ray Scattering Analysis of Temperature Effects on Self-Assembling / Mariani, Paolo; Spinozzi, Francesco; F., Federiconi; Ortore, Maria Grazia; H., Amenitsch; L., Spindler; I., DREVENSEK OLENIK. - In: JOURNAL OF NUCLEIC ACIDS. - ISSN 2090-0201. - ELETTRONICO. - 2010:(2010), p. ID 472478. [10.4061/2010/472478]

Guanosine Quadruplexes in Solution: A Small-Angle X-ray Scattering Analysis of Temperature Effects on Self-Assembling

MARIANI, Paolo;SPINOZZI, Francesco;ORTORE, Maria Grazia;
2010-01-01

Abstract

We investigated quadruplex formation in aqueous solutions of 2′ -deoxyriboguanosine 5′ -monophosphate, d(pG), which takes place in the absence of the covalent axial backbone. A series of in-solution small angle X-ray scattering experiments on d(pG) have been performed as a function of temperature in the absence of excess salt, at a concentration just above the critical one at which self-assembling occurs. A global fit approach has been used to derive composition and size distribution of the scattering particles as a function of temperature. The obtained results give thermodynamical justification for the observed phase-behavior, indicating that octamer formation is essential for quadruplex elongation. Our investigation shows that d(pG) quadruplexes are very suitable to assess the potential of G-quadruplex formation and to study the self-assembling thermodynamics.
2010
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11566/33169
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