The role of CD4+ T cells in the pathogenesis of aplastic anemia (AA) is not well characterized. We investigate CD4+ T-cell subsets in AA. Sixty-three patients with acquired AA were studied. Th1 and Th2 cells were significantly higher in AA patients than in healthy donors (HDs; P = .03 and P = .006). Tregs were significantly lower in patients with severe AA than in HDs (P < .001) and patients with nonsevere AA (P = .01). Th17 cells were increased in severeAA(P = .02) but normal in non-severe AA. Activated and resting Tregs were reduced in AA (P = .004; P = .01), whereas cytokine-secreting non-Tregs were increased (P = .003). Tregs from AA patients were unable to suppress normal effector T cells. In contrast, AA effector T cells were suppressible by Tregs from HDs. Th1 clonality in AA, investigated by high-throughput sequencing, was greater than in HDs (P = .03). Our results confirm that Th1 and Th2 cells are expanded and Tregs are functionally abnormal in AA. The clonally restricted expansion of Th1 cells is most likely to be antigen-driven, and induces an inflammatory environment, that exacerbate the functional impairment of Tregs, which are reduced in number. © 2012 by The American Society of Hematology.

Functional characterization of CD4+ T cells in aplastic anemia / Kordasti, S.; Marsh, J.; Al-Khan, S.; Jiang, J.; Smith, A.; Mohamedali, A.; Abellan, P. P.; Veen, C.; Costantini, B.; Kulasekararaj, A. G.; Benson-Quarm, N.; Seidl, T.; Mian, S. A.; Farzaneh, F.; Mufti, G. J.. - In: BLOOD. - ISSN 0006-4971. - 119:9(2012), pp. 2033-2043. [10.1182/blood-2011-08-368308]

Functional characterization of CD4+ T cells in aplastic anemia

Kordasti S.
Primo
Formal Analysis
;
2012-01-01

Abstract

The role of CD4+ T cells in the pathogenesis of aplastic anemia (AA) is not well characterized. We investigate CD4+ T-cell subsets in AA. Sixty-three patients with acquired AA were studied. Th1 and Th2 cells were significantly higher in AA patients than in healthy donors (HDs; P = .03 and P = .006). Tregs were significantly lower in patients with severe AA than in HDs (P < .001) and patients with nonsevere AA (P = .01). Th17 cells were increased in severeAA(P = .02) but normal in non-severe AA. Activated and resting Tregs were reduced in AA (P = .004; P = .01), whereas cytokine-secreting non-Tregs were increased (P = .003). Tregs from AA patients were unable to suppress normal effector T cells. In contrast, AA effector T cells were suppressible by Tregs from HDs. Th1 clonality in AA, investigated by high-throughput sequencing, was greater than in HDs (P = .03). Our results confirm that Th1 and Th2 cells are expanded and Tregs are functionally abnormal in AA. The clonally restricted expansion of Th1 cells is most likely to be antigen-driven, and induces an inflammatory environment, that exacerbate the functional impairment of Tregs, which are reduced in number. © 2012 by The American Society of Hematology.
2012
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11566/328523
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