Abstract: Three dimensional scaffolds microfabricated using pressure-assisted microsyringe (PAM) with controlled geometry and porous membranes obtained using salt leaching were both tested with three different cell types to identify an optimal microstructural architecture for tissue engineering. MG63 (osteoblast-like cells) were used as models of mesenchymal bone tissue and human endothelial cells and NCTC2544 (keratinocytes) represented two epithelial tissues. Both porosity and stiffness of PLGA structures were measured, and cell morphology and cytoskeletal organization analyzed using SEM and actin labeling. The results show that overall the PAM scaffolds, which have a repeated and regular microstructure, are more biocompatible than the random pore salt-leached membranes, and that surface morphology as well as substrate stiffness modulates cell behavior. 2007 Wiley Periodicals, Inc. J Biomed Mater Res 85A: 466–476, 2008.
Rapid-prototyped and salt-leached PLGA scaffolds condition cell morpho-functional behavior / MATTIOLI BELMONTE CIMA, Monica; Vozzi, G.; Kyriakidou, K.; Pulieri, E.; Lucarini, Guendalina; Vinci, B.; Pugnaloni, Armanda; Biagini, G.; Ahluwalia, A.. - In: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A. - ISSN 1552-4965. - STAMPA. - 85 (2):(2008), pp. 466-476. [10.1002/jbm.a.31483]
Rapid-prototyped and salt-leached PLGA scaffolds condition cell morpho-functional behavior.
MATTIOLI BELMONTE CIMA, Monica;LUCARINI, Guendalina;PUGNALONI, Armanda;
2008-01-01
Abstract
Abstract: Three dimensional scaffolds microfabricated using pressure-assisted microsyringe (PAM) with controlled geometry and porous membranes obtained using salt leaching were both tested with three different cell types to identify an optimal microstructural architecture for tissue engineering. MG63 (osteoblast-like cells) were used as models of mesenchymal bone tissue and human endothelial cells and NCTC2544 (keratinocytes) represented two epithelial tissues. Both porosity and stiffness of PLGA structures were measured, and cell morphology and cytoskeletal organization analyzed using SEM and actin labeling. The results show that overall the PAM scaffolds, which have a repeated and regular microstructure, are more biocompatible than the random pore salt-leached membranes, and that surface morphology as well as substrate stiffness modulates cell behavior. 2007 Wiley Periodicals, Inc. J Biomed Mater Res 85A: 466–476, 2008.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
