The aim of this study was to determine the excretion of methylone and its metabolites in sweat following the ingestion of increasing controlled doses of 50, 100, 150 and 200 mg of methylone to twelve healthy volunteers involved in a clinical trial. Methylone and its metabolites 4-hydroxy-3-methoxy-N-methylcathinone (HMMC) and 3,4-methylenedioxycathinone (MDC) were analyzed in sweat patches by liquid chromatography-tandem mass spectrometry. Methylone and MDC were detected in sweat at 2 h and reached their highest accumulation (C-max) at 24 h after the administration of 50, 100, 150 and 200 mg doses. In contrast, HMMC was not detectable at any time interval after each dose. Sweat proved to be a suitable matrix for methylone and its metabolites' determination in clinical and toxicological studies, providing a concentration that reveals recent drug consumption.
Sweat Testing for the Detection of Methylone after Controlled Administrations in Humans / Di Giorgi, Alessandro; Sprega, Giorgia; Poyatos, Lourdes; Papaseit, Esther; Pérez-Mañá, Clara; Di Trana, Annagiulia; Varì, Maria Rosaria; Busardo', Francesco Paolo; Pichini, Simona; Zaami, Simona; Lo Faro, Alfredo Fabrizio; Farré, Magí. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 24:8(2023), p. 7395. [10.3390/ijms24087395]
Sweat Testing for the Detection of Methylone after Controlled Administrations in Humans
Di Giorgi, Alessandro;Sprega, Giorgia;Di Trana, Annagiulia;Busardo', Francesco Paolo;Pichini, Simona;Lo Faro, Alfredo Fabrizio;
2023-01-01
Abstract
The aim of this study was to determine the excretion of methylone and its metabolites in sweat following the ingestion of increasing controlled doses of 50, 100, 150 and 200 mg of methylone to twelve healthy volunteers involved in a clinical trial. Methylone and its metabolites 4-hydroxy-3-methoxy-N-methylcathinone (HMMC) and 3,4-methylenedioxycathinone (MDC) were analyzed in sweat patches by liquid chromatography-tandem mass spectrometry. Methylone and MDC were detected in sweat at 2 h and reached their highest accumulation (C-max) at 24 h after the administration of 50, 100, 150 and 200 mg doses. In contrast, HMMC was not detectable at any time interval after each dose. Sweat proved to be a suitable matrix for methylone and its metabolites' determination in clinical and toxicological studies, providing a concentration that reveals recent drug consumption.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
