Two bacterial strainsLactobacillus fructivorans(AS17B), isolated from adult seabream (Sparus aurataL.) gut, andLactoba-cillus plantarum(906), isolated from human faeces, were administered contemporaneously during seabream development using Brachionus plicatilisand/orArtemia salinaand dry feed as vectors. Experimental group A received the probiotic strains already via rotifers from day 5 post-hatch (ph), whereas treatment of group B began withArtemiafeeding from day 27 ph. Fish were sampled at day 28 ph (group A and control) and day 99 ph (groups A, B and control) for electron microscopy, histology and immunohistochemistry with the polyclonal antiserum ORa against homologous serum Ig and the mAb G7 specific for seabream acidophilic granulocytes. In all groups, timing and pattern of differentiation of the digestive tract did not differ. Furthermore, neither tissue damage nor manifest inflammation was provoked by probiotic adminis-tration. At day 28 ph, the developing GALT already housed mucosal leucocytes, including Ig þ cells but no acidophilic granulocytes. No differences were seen between experimental groups. At day 99 ph, the density of Ig þ cells (þ51%) and acidophilic granulocytes (þ284%) was significantly higher (p<0.05) in group A than in controls. Also group B had a higher density of Ig þ cells (þ17%) and acidophilic granulocytes (þ130%) compared with controls, although less pronounced. Light and electron microscopy observations detailed the occurrence of heterogeneous pop-ulations of lymphocytes and granulocytes in the developing intestinal mucosa, and highlighted the net expansion of G7 þ acidophilic granulocytes (Aþ536%, Bþ292% vs. control) due to probiotic administration.

Effects of administration of probiotic strains on GALT of larval gilthead seabream: immunohistochemical and ultrastructural studies / Picchietti, S.; Mazzini, M.; Taddei, A. R.; Renna, R.; Fausto, A. M.; Mulero, V.; Carnevali, Oliana; Cresci, A.; Abelli, L.. - In: FISH AND SHELLFISH IMMUNOLOGY. - ISSN 1050-4648. - 22:(2007), pp. 57-67.

Effects of administration of probiotic strains on GALT of larval gilthead seabream: immunohistochemical and ultrastructural studies

CARNEVALI, Oliana;
2007-01-01

Abstract

Two bacterial strainsLactobacillus fructivorans(AS17B), isolated from adult seabream (Sparus aurataL.) gut, andLactoba-cillus plantarum(906), isolated from human faeces, were administered contemporaneously during seabream development using Brachionus plicatilisand/orArtemia salinaand dry feed as vectors. Experimental group A received the probiotic strains already via rotifers from day 5 post-hatch (ph), whereas treatment of group B began withArtemiafeeding from day 27 ph. Fish were sampled at day 28 ph (group A and control) and day 99 ph (groups A, B and control) for electron microscopy, histology and immunohistochemistry with the polyclonal antiserum ORa against homologous serum Ig and the mAb G7 specific for seabream acidophilic granulocytes. In all groups, timing and pattern of differentiation of the digestive tract did not differ. Furthermore, neither tissue damage nor manifest inflammation was provoked by probiotic adminis-tration. At day 28 ph, the developing GALT already housed mucosal leucocytes, including Ig þ cells but no acidophilic granulocytes. No differences were seen between experimental groups. At day 99 ph, the density of Ig þ cells (þ51%) and acidophilic granulocytes (þ284%) was significantly higher (p<0.05) in group A than in controls. Also group B had a higher density of Ig þ cells (þ17%) and acidophilic granulocytes (þ130%) compared with controls, although less pronounced. Light and electron microscopy observations detailed the occurrence of heterogeneous pop-ulations of lymphocytes and granulocytes in the developing intestinal mucosa, and highlighted the net expansion of G7 þ acidophilic granulocytes (Aþ536%, Bþ292% vs. control) due to probiotic administration.
2007
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11566/31334
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 128
  • ???jsp.display-item.citation.isi??? ND
social impact