Abstract. This research developed a simple and not expensive DNA method for the qualitative identification of plant raw materials used as feed mixtures. Specific simple sequence tagged (STS) markers were developed to detect faba bean (Lectin A gene), field pea (Convicilin A gene), grain sorghum (UDP-glucosyltransferase gene) and barley (Hordoindoline-a gene), whereas identification of durum and common wheat (lipid transfer protein gene), soybean (Gly m Bd 30K allergen gene) and maize (invertase gene) was carried out using markers available from the literature. Cross-reactivity of the primer pairs was also checked against oat, rye, kidney bean and lentil. The method was effectively applied to the analysis of four mixtures and extruded feedstuff. It could be included in traceability and certification of animal feeding systems within high quality animal production chains which are strictly related to the production area by the valorisation of locally grown raw materials.
A DNA method for qualitative identification of raw materials in feedstuff / Tavoletti, S.; Iommarini, L.; Pasquini, M.. - In: EUROPEAN FOOD RESEARCH AND TECHNOLOGY. - ISSN 1438-2377. - 229:3(2009), pp. 475-484. [10.1007/s00217-009-1077-1]
A DNA method for qualitative identification of raw materials in feedstuff.
Tavoletti S.;Iommarini L.;Pasquini M.
2009-01-01
Abstract
Abstract. This research developed a simple and not expensive DNA method for the qualitative identification of plant raw materials used as feed mixtures. Specific simple sequence tagged (STS) markers were developed to detect faba bean (Lectin A gene), field pea (Convicilin A gene), grain sorghum (UDP-glucosyltransferase gene) and barley (Hordoindoline-a gene), whereas identification of durum and common wheat (lipid transfer protein gene), soybean (Gly m Bd 30K allergen gene) and maize (invertase gene) was carried out using markers available from the literature. Cross-reactivity of the primer pairs was also checked against oat, rye, kidney bean and lentil. The method was effectively applied to the analysis of four mixtures and extruded feedstuff. It could be included in traceability and certification of animal feeding systems within high quality animal production chains which are strictly related to the production area by the valorisation of locally grown raw materials.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.