Ciliary neurotrophic factor (CNTF) is a pleiotropic cytokine that signals through a receptor complex containing a specific subunit, CNTF receptor α (CNTFRα). The two molecules are constitutively expressed in key structures for human placental growth and differentiation. The possible role of CNTF in enhancing cell proliferation and/or invasion during placental development and remodelling was investigated using HTR-8/SVneo and BeWo cells, taken respectively as cytotrophoblast and syncytiotrophoblast models. In both cell lines, treatment with human recombinant (hr) CNTF activated JAK2/STAT3 signalling and inhibited the ERK pathway. Interestingly, in HTR-8/SVneo cells, 50 ng hrCNTF induced significant downregulation of matrix metalloprotease (MMP)-1 and significant upregulation of MMP-9. Moreover, pharmacological inhibition of JAK2/STAT3 signalling by AG490 and curcumin resulted in MMP-9 downregulation; it activated the ERK signalling pathway and upregulated MMP-1 expression. Collectively, these data suggest a role for CNTF signalling in extravillous cytotrophoblast invasion through the modulation of specific MMPs.
Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development / Tossetta, Giovanni; Fantone, Sonia; MARINELLI BUSILACCHI, Elena; Di Simone, Nicoletta; R Giannubilo, Stefano; Scambia, Giovanni; Giordano, Antonio; Marzioni, Daniela. - In: CELL & TISSUE RESEARCH. - ISSN 1432-0878. - (2022). [10.1007/s00441-022-03658-1]