Recently, the demineralized dentin matrix has been suggested as an alternative material to autologous bone grafts and xenografts for clinical purposes. The aim of this study was to investi-gate the effect of different times of demineralization on the chemical composition and the surface morphology of dentinal particles. Extracted teeth were ground and divided into 5 groups based on demineralization time (T0 = 0 min, T2 = 2 min, T5 = 5 min, T10 = 10 min, and T60 = 60 min) with 12% EDTA. The analysis was performed using Fourier-Transform Mid-Infrared spectroscopy (FT-MIR) and Scanning Electron Microscopy (SEM) (p < 0.05). The FT-MIR analysis showed a progressive reduction of the concentration of both PO43− and CO32− in the specimens (T0 > T2 > T5 > T10 > T60). On the contrary, the organic (protein) component did not undergo any change. The SEM examination showed that increasing the times of demineralization resulted in a smoother surface of the dentin particles and a higher number of dentinal tubules.

SEM and FT-MIR Analysis of Human Demineralized Dentin Matrix: An In Vitro Study / Meme, L.; Strappa, E. M.; Monterubbianesi, R.; Bambini, F.; Mummolo, S.. - In: APPLIED SCIENCES. - ISSN 2076-3417. - 12:3(2022). [10.3390/app12031480]

SEM and FT-MIR Analysis of Human Demineralized Dentin Matrix: An In Vitro Study

Meme L.
;
Monterubbianesi R.;Bambini F.;
2022-01-01

Abstract

Recently, the demineralized dentin matrix has been suggested as an alternative material to autologous bone grafts and xenografts for clinical purposes. The aim of this study was to investi-gate the effect of different times of demineralization on the chemical composition and the surface morphology of dentinal particles. Extracted teeth were ground and divided into 5 groups based on demineralization time (T0 = 0 min, T2 = 2 min, T5 = 5 min, T10 = 10 min, and T60 = 60 min) with 12% EDTA. The analysis was performed using Fourier-Transform Mid-Infrared spectroscopy (FT-MIR) and Scanning Electron Microscopy (SEM) (p < 0.05). The FT-MIR analysis showed a progressive reduction of the concentration of both PO43− and CO32− in the specimens (T0 > T2 > T5 > T10 > T60). On the contrary, the organic (protein) component did not undergo any change. The SEM examination showed that increasing the times of demineralization resulted in a smoother surface of the dentin particles and a higher number of dentinal tubules.
2022
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11566/299125
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