Background: LL-37 is the only human antimicrobial peptide that belongs to the cathelici-dins. The aim of the study was to evaluate the efficacy of LL-37 in the management of MRSA-in-fected surgical wounds in mice. Methods: A wound on the back of adult male BALB/c mice was made and inoculated with Staphylococcus aureus. Two control groups were formed (uninfected and not treated, C0; infected and not treated, C1) and six contaminated groups were treated, respec-tively, with: teicoplanin, LL-37, given topically and /or systemically. Histological examination of VEGF expression and micro-vessel density, and bacterial cultures of wound tissues, were per-formed. Results: Histological examination of wounds in the group treated with topical and intra-peritoneal LL-37 showed increased re-epithelialization, formation of the granulation tissue, collagen organization, and angiogenesis. Conclusions: Based on the mode of action, LL-37 has a potential future role in the management of infected wounds.
Efficacy of cathelicidin ll-37 in an mrsa wound infection mouse model
Simonetti O.;Cirioni O.;Goteri G.;Lucarini G.;Molinelli E.;Morroni G.;Ghiselli R.;Giacometti A.;Offidani A.
2021-01-01
Abstract
Background: LL-37 is the only human antimicrobial peptide that belongs to the cathelici-dins. The aim of the study was to evaluate the efficacy of LL-37 in the management of MRSA-in-fected surgical wounds in mice. Methods: A wound on the back of adult male BALB/c mice was made and inoculated with Staphylococcus aureus. Two control groups were formed (uninfected and not treated, C0; infected and not treated, C1) and six contaminated groups were treated, respec-tively, with: teicoplanin, LL-37, given topically and /or systemically. Histological examination of VEGF expression and micro-vessel density, and bacterial cultures of wound tissues, were per-formed. Results: Histological examination of wounds in the group treated with topical and intra-peritoneal LL-37 showed increased re-epithelialization, formation of the granulation tissue, collagen organization, and angiogenesis. Conclusions: Based on the mode of action, LL-37 has a potential future role in the management of infected wounds.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
