Melanoma accounts for less than 5% of all cutaneous neoplasms but is responsible for the greater part of skin cancer-related deaths. Therefore, the identification of molecules that could serve as the therapeutic target is urgent. This study focused on the enzyme nicotinamide N-methyltransferase (NNMT). The effect of NNMT knockdown on cell proliferation and migration of A375 melanoma cells was evaluated by MTT and wound healing assays, respectively. Viability of A375 cells downregulating NNMT was also explored under treatment with dacarbazine, a chemotherapeutic drug approved for advanced melanoma treatment. The impact of enzyme knockdown on cell proliferation and chemosensitivity was also investigated in WM-115 melanoma cells. Results obtained demonstrated that NNMT silencing led to a significant reduction of cell proliferation and migration of A375 cells. Moreover, enzyme downregulation was associated with an increase of melanoma cells sensitivity to treatment with dacarbazine. Analogous effects induced by enzyme knockdown on cell proliferation and chemosensitivity were also found in the WM-115 cell line. Our data seem to demonstrate that NNMT could represent a promising molecular target for the effective treatment of this form of skin cancer.

Nicotinamide N-methyltransferase gene silencing enhances chemosensitivity of melanoma cell lines / Campagna, R; Salvolini, E; Pompei, V; Pozzi, V; Salvucci, A; Molinelli, E; Brisigotti, V; Sartini, D; Campanati, A; Offidani, A; Emanuelli, M. - In: PIGMENT CELL & MELANOMA RESEARCH. - ISSN 1755-148X. - STAMPA. - 34:6(2021), pp. 1039-1048. [10.1111/pcmr.12993]

Nicotinamide N-methyltransferase gene silencing enhances chemosensitivity of melanoma cell lines

Campagna R
Primo
;
Salvolini E;Pompei V;Pozzi V;Salvucci A;Molinelli E;Brisigotti V;Sartini D
;
Campanati A;Offidani A;Emanuelli M
Ultimo
2021-01-01

Abstract

Melanoma accounts for less than 5% of all cutaneous neoplasms but is responsible for the greater part of skin cancer-related deaths. Therefore, the identification of molecules that could serve as the therapeutic target is urgent. This study focused on the enzyme nicotinamide N-methyltransferase (NNMT). The effect of NNMT knockdown on cell proliferation and migration of A375 melanoma cells was evaluated by MTT and wound healing assays, respectively. Viability of A375 cells downregulating NNMT was also explored under treatment with dacarbazine, a chemotherapeutic drug approved for advanced melanoma treatment. The impact of enzyme knockdown on cell proliferation and chemosensitivity was also investigated in WM-115 melanoma cells. Results obtained demonstrated that NNMT silencing led to a significant reduction of cell proliferation and migration of A375 cells. Moreover, enzyme downregulation was associated with an increase of melanoma cells sensitivity to treatment with dacarbazine. Analogous effects induced by enzyme knockdown on cell proliferation and chemosensitivity were also found in the WM-115 cell line. Our data seem to demonstrate that NNMT could represent a promising molecular target for the effective treatment of this form of skin cancer.
2021
File in questo prodotto:
File Dimensione Formato  
Campagna_Nicotinamide-N-methyltransferase_2021.pdf

Solo gestori archivio

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza d'uso: Tutti i diritti riservati
Dimensione 951.35 kB
Formato Adobe PDF
951.35 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
pcmr.pdf

Open Access dal 30/05/2022

Descrizione: This is the peer reviewed version of the following article: Nicotinamide N-methyltransferase gene silencing enhances chemosensitivity of melanoma cell lines / Campagna, R; Salvolini, E; Pompei, V; Pozzi, V; Salvucci, A; Molinelli, E; Brisigotti, V; Sartini, D; Campanati, A; Offidani, A; Emanuelli, M. - In: PIGMENT CELL & MELANOMA RESEARCH. - ISSN 1755-148X. - STAMPA. - 34:6(2021), pp. 1039-1048. © 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd . Published by John Wiley & Sons Ltd, which has been published in final form at https://dx.doi.org/10.1111/pcmr.12993. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions https://authorservices.wiley.com/author-resources/Journal-Authors/licensing/self-archiving.html. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Tipologia: Documento in post-print (versione successiva alla peer review e accettata per la pubblicazione)
Licenza d'uso: Licenza specifica dell’editore (allegare)
Dimensione 937.54 kB
Formato Adobe PDF
937.54 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11566/290822
Citazioni
  • ???jsp.display-item.citation.pmc??? 23
  • Scopus 31
  • ???jsp.display-item.citation.isi??? 30
social impact