The essential oils of Origanum ehrenbergii and O. syriacum collected in Lebanon were analysed by GC and GC-MS and evaluated for their anticholinesterase, NO production inhibitory activities, and antioxidant properties. O. ehrenbergi essential oil was characterised by the presence of 37 components, representing 94.9% of the total oil of which thymol (19%) and p-cymene (16.1%) were the main abundant compounds. Thirty-six compounds characterised the O. syriacum essential oil, representing 90.6% of the total oil. The most abundant components were thymol (24.7%) and carvacrol (17.6%). O. ehrenbergii demonstrated interesting scavenging effects on DPPH with an IC(50) value of 0.99 mu g/ml. In addition, both O. ehrenbergii and O. syniacum oils inhibited oxidation of linoleic acid after 30 min of incubation, as well as after 60 min of incubation with IC(50) values of 42.1 and 33.6 mu g/ml, and 46.9 and 58.9 mu g/ml, respectively. Interestingly, O. ehrenbergii oil inhibited NO production in the murine monocytic macrophage cell line RAW 264.7 with an IC(50) value of 66.4 mu g/ml. Acetylcholinesterase (ACH) and butyrylcholinesterase (BChE) inhibition was assessed by modifications of the Ellman's method. O. ehrenbergii exhibited a strong activity against both cholinesterases with IC(50) values of 0.3 mu g/ml. The data suggest that O. ehrenbergii and O. syriacum oils could be used as a valuable new flavour with functional properties for food or nutriceutical products with particular relevance to supplements for the elderly
Chemical analysis, antioxidant, anti inflammatory and anticholinesterase activities of Origanum ehrenbergii Boiss and Origanum syriacum L. essential oils / Loizzo, Mr; Menichini, F; Conforti, F; Tundis, R; Bonesi, M; Saab, Am; Statti, Ga; DE CINDIO, B; Houghton, Pj; Menichini, F; Frega, Natale Giuseppe. - In: FOOD CHEMISTRY. - ISSN 0308-8146. - 117:(2009), pp. 174-180.
Chemical analysis, antioxidant, anti inflammatory and anticholinesterase activities of Origanum ehrenbergii Boiss and Origanum syriacum L. essential oils
FREGA, Natale Giuseppe
2009-01-01
Abstract
The essential oils of Origanum ehrenbergii and O. syriacum collected in Lebanon were analysed by GC and GC-MS and evaluated for their anticholinesterase, NO production inhibitory activities, and antioxidant properties. O. ehrenbergi essential oil was characterised by the presence of 37 components, representing 94.9% of the total oil of which thymol (19%) and p-cymene (16.1%) were the main abundant compounds. Thirty-six compounds characterised the O. syriacum essential oil, representing 90.6% of the total oil. The most abundant components were thymol (24.7%) and carvacrol (17.6%). O. ehrenbergii demonstrated interesting scavenging effects on DPPH with an IC(50) value of 0.99 mu g/ml. In addition, both O. ehrenbergii and O. syniacum oils inhibited oxidation of linoleic acid after 30 min of incubation, as well as after 60 min of incubation with IC(50) values of 42.1 and 33.6 mu g/ml, and 46.9 and 58.9 mu g/ml, respectively. Interestingly, O. ehrenbergii oil inhibited NO production in the murine monocytic macrophage cell line RAW 264.7 with an IC(50) value of 66.4 mu g/ml. Acetylcholinesterase (ACH) and butyrylcholinesterase (BChE) inhibition was assessed by modifications of the Ellman's method. O. ehrenbergii exhibited a strong activity against both cholinesterases with IC(50) values of 0.3 mu g/ml. The data suggest that O. ehrenbergii and O. syriacum oils could be used as a valuable new flavour with functional properties for food or nutriceutical products with particular relevance to supplements for the elderlyI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
