Routine culture-based diagnosis of Pseudomonas aeruginosa lung infection in Cystic Fibrosis (CF) patients can be hampered by the phenotypic variability of the microorganism, including its transition to a Viable But Non-Culturable (VBNC) state. The aim of this study was to validate an ecfX-targeting qPCR protocol developed to detect all viable P. aeruginosa bacteria and to identify VBNC forms in CF sputum samples.
Detection of viable but non-culturable Pseudomonas aeruginosa in cystic fibrosis by qPCR: a validation study / Mangiaterra, Gianmarco; Amiri, Mehdi; Di Cesare, Andrea; Pasquaroli, Sonia; Manso, Esther; Cirilli, Natalia; Citterio, Barbara; Vignaroli, Carla; Biavasco, Francesca. - In: BMC INFECTIOUS DISEASES. - ISSN 1471-2334. - STAMPA. - 18:1(2018), pp. 701-707. [10.1186/s12879-018-3612-9]
Detection of viable but non-culturable Pseudomonas aeruginosa in cystic fibrosis by qPCR: a validation study
Mangiaterra, Gianmarco
;Vignaroli, Carla;Biavasco, Francesca
2018-01-01
Abstract
Routine culture-based diagnosis of Pseudomonas aeruginosa lung infection in Cystic Fibrosis (CF) patients can be hampered by the phenotypic variability of the microorganism, including its transition to a Viable But Non-Culturable (VBNC) state. The aim of this study was to validate an ecfX-targeting qPCR protocol developed to detect all viable P. aeruginosa bacteria and to identify VBNC forms in CF sputum samples.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
