Routine culture-based diagnosis of Pseudomonas aeruginosa lung infection in Cystic Fibrosis (CF) patients can be hampered by the phenotypic variability of the microorganism, including its transition to a Viable But Non-Culturable (VBNC) state. The aim of this study was to validate an ecfX-targeting qPCR protocol developed to detect all viable P. aeruginosa bacteria and to identify VBNC forms in CF sputum samples.
Detection of viable but non-culturable Pseudomonas aeruginosa in cystic fibrosis by qPCR: a validation study / Mangiaterra, G., Amiri, M., Di Cesare, A., Pasquaroli, S., Manso, E., Cirilli, N., Citterio, B., Vignaroli, C., Biavasco, F.. - In: BMC INFECTIOUS DISEASES. - ISSN 1471-2334. - STAMPA. - 18:1(2018), pp. 701-707. [10.1186/s12879-018-3612-9]
Detection of viable but non-culturable Pseudomonas aeruginosa in cystic fibrosis by qPCR: a validation study
Mangiaterra, Gianmarco
;Vignaroli, Carla;Biavasco, Francesca
2018-01-01
Abstract
Routine culture-based diagnosis of Pseudomonas aeruginosa lung infection in Cystic Fibrosis (CF) patients can be hampered by the phenotypic variability of the microorganism, including its transition to a Viable But Non-Culturable (VBNC) state. The aim of this study was to validate an ecfX-targeting qPCR protocol developed to detect all viable P. aeruginosa bacteria and to identify VBNC forms in CF sputum samples.| File | Dimensione | Formato | |
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