In this study, IMO-TaqI satDNA, previously isolated in several species of Lacertidae, was isolated and characterized from four species of the genus Lacerta and three of the genus Timon. The aim was to gain further insights into the evolutionary dynamics of this satDNA, its occurrence among lacertids and to understand if it plays any role in sex chromosome evolution in these seven species. The results here obtained highlighted the presence of this repetitive element in the genome of all the species investigated, thus indicating that IMO-TaqI satDNA is evolutionary conserved among a wide variety of lacertids. In addition, this element was found to be very abundant in the constitutive heterochromatin of the W-sex chromosome of the four Lacerta species investigated. The occurrence of IMO-TaqI satDNA on Lacerta heterochromosome suggests that it is involved in the differentiation of the W chromosome by heterochromatinization, and the fact that it is absent in the W of other lacertids investigated seems to confirm that repetitive DNA sequences would remain randomly trapped into the sex chromosomes, undergoing amplification as a consequence of the suppression of recombination.

Characterization of a satellite DNA in the genera Lacerta and Timon (Reptilia, Lacertidae) and its role in the differentiation of the W chromosome / Giovannotti, Massimo; Nisi Cerioni, Paola; Rojo, Verónica; Olmo, Ettore; Slimani, Tahar; Splendiani, Andrea; Caputo Barucchi, Vincenzo. - In: JOURNAL OF EXPERIMENTAL ZOOLOGY. PART B, MOLECULAR AND DEVELOPMENTAL EVOLUTION.. - ISSN 1552-5007. - ELETTRONICO. - (2018). [10.1002/jez.b.22790]

Characterization of a satellite DNA in the genera Lacerta and Timon (Reptilia, Lacertidae) and its role in the differentiation of the W chromosome

Giovannotti, Massimo
Membro del Collaboration Group
;
Nisi Cerioni, Paola
Membro del Collaboration Group
;
Olmo, Ettore
Membro del Collaboration Group
;
Splendiani, Andrea
Membro del Collaboration Group
;
Caputo Barucchi, Vincenzo
Membro del Collaboration Group
2018-01-01

Abstract

In this study, IMO-TaqI satDNA, previously isolated in several species of Lacertidae, was isolated and characterized from four species of the genus Lacerta and three of the genus Timon. The aim was to gain further insights into the evolutionary dynamics of this satDNA, its occurrence among lacertids and to understand if it plays any role in sex chromosome evolution in these seven species. The results here obtained highlighted the presence of this repetitive element in the genome of all the species investigated, thus indicating that IMO-TaqI satDNA is evolutionary conserved among a wide variety of lacertids. In addition, this element was found to be very abundant in the constitutive heterochromatin of the W-sex chromosome of the four Lacerta species investigated. The occurrence of IMO-TaqI satDNA on Lacerta heterochromosome suggests that it is involved in the differentiation of the W chromosome by heterochromatinization, and the fact that it is absent in the W of other lacertids investigated seems to confirm that repetitive DNA sequences would remain randomly trapped into the sex chromosomes, undergoing amplification as a consequence of the suppression of recombination.
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Descrizione: This is the pre-peer reviewed version of the following article: Massimo Giovannotti, Paola Nisi Cerioni, Verónica Rojo, Ettore Olmo, Tahar Slimani, Andrea Splendiani, Vincenzo Caputo Barucchi - Characterization of a satellite DNA in the genera Lacerta and Timon (Reptilia, Lacertidae) and its role in the differentiation of the W chromosome - JEZ-B Molecular and Developmental Evolution - 2018, which has been published in final form at https://dx.doi.org/10.1002/jez.b.22790.This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11566/253289
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