The present study was designed to achieve a comprehensive analysis of gender-related differences in themyoelectric activity of lower limb muscles during normal walking at self-selected speed and cadence,in terms of muscle activation patterns and occurrence frequencies. To this aim, statistical gait analysis(SGA) of surface EMG signal from tibialis anterior (TA), gastrocnemius lateralis (GL), rectus femoris (RF),biceps femoris (BF) and vastus lateralis (VL) was performed in 11 female (F-group) and 11 male (M-group)age-matched healthy young adults. SGA is a recent methodology performing a statistical characteriza-tion of gait, by averaging spatio-temporal and sEMG-based parameters over numerous strides. Findingsshowed that males and females walk at the same comfortable speed, despite the significantly lowerheight and higher cadence detected in females. No significant differences in muscle onset/offset weredetected between groups. The analysis of occurrence frequencies of muscle activity showed no signif-icant differences in BF and RF, between groups. Conversely, in F-group, compared with M-group, GL,TA and VL showed a significantly higher occurrence frequency in the modalities with a high number ofactivations, and a significantly lower occurrence frequency in the modalities with a low number of acti-vations. These findings indicate a propensity of females for a more complex recruitment of TA, GL and VLduring walking, compared to males. The observed differences recommend the suitability of developingelectromyographic databases, separated for males and females.
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