Asbestos exposure and malignant lung disease are epidemiologically associated. Mineral fibres are still objects of multidisciplinary and intensive investigations as their cyto and genotoxic damages pathways remain poorly understood. Methods Human mesothelial cells (MeT5A) were exposed to mineralogically characterized asbestos fibres: UICC chrysotile, widely used in the production of asbestos containing matherial; UICC crocidolite, well known for its carcinogenic power; erionite from Jersy Nevada (USA) [1] , chrysotile from Valmalenco (Central Alps, Italy) [2,3], both naturally occurring and airborn asbestos fibres. Citotoxic and genotoxic effects were investigated by MTT test and DNA Double-Strand Breaks [4] respectively and compared. Discussion and Results Both MTT test (Fig.1) and immunocytochemical detection of phosphorilated Histone H2AX indicated cytotoxicity and genotoxic cancerogen potential of all fibres, including airborn fibres from natural expositions, related to their chemical and structural features [3]. [1] Pollastri et al. (2016) Chemosphere, 164, 547-557. [2] Turci et al. (2010) J. Toxicol. Environ. Health A, 73, 368- 377. [3] Gualtieri (2018) Toxicol. App. Pharmacol. 361, 89- 98. [4] Msiska et al. (2010) Am J Respir Cell Mol Biol. 43, 210–219.
Asbestos induced toxicity: in vitro different effects of different fibres / DI VALERIO, S.; Ramini, D.; Mensà, E.; Fazioli, F.; Marzioni, D.; Gualtieri, A. F.; Pugnaloni, A.. - ELETTRONICO. - (2020), p. reference number gold2020:abs:2020002118. (Intervento presentato al convegno GOLDSCHMIDT 2020 tenutosi a Hawai'i Honolulu nel 21-26 June 2020).
Asbestos induced toxicity: in vitro different effects of different fibres.
S. DI VALERIOPrimo
Membro del Collaboration Group
;D. RAMINISecondo
Membro del Collaboration Group
;F. FAZIOLIMembro del Collaboration Group
;D. MARZIONIMembro del Collaboration Group
;A. PUGNALONI
Ultimo
Writing – Original Draft Preparation
2020-01-01
Abstract
Asbestos exposure and malignant lung disease are epidemiologically associated. Mineral fibres are still objects of multidisciplinary and intensive investigations as their cyto and genotoxic damages pathways remain poorly understood. Methods Human mesothelial cells (MeT5A) were exposed to mineralogically characterized asbestos fibres: UICC chrysotile, widely used in the production of asbestos containing matherial; UICC crocidolite, well known for its carcinogenic power; erionite from Jersy Nevada (USA) [1] , chrysotile from Valmalenco (Central Alps, Italy) [2,3], both naturally occurring and airborn asbestos fibres. Citotoxic and genotoxic effects were investigated by MTT test and DNA Double-Strand Breaks [4] respectively and compared. Discussion and Results Both MTT test (Fig.1) and immunocytochemical detection of phosphorilated Histone H2AX indicated cytotoxicity and genotoxic cancerogen potential of all fibres, including airborn fibres from natural expositions, related to their chemical and structural features [3]. [1] Pollastri et al. (2016) Chemosphere, 164, 547-557. [2] Turci et al. (2010) J. Toxicol. Environ. Health A, 73, 368- 377. [3] Gualtieri (2018) Toxicol. App. Pharmacol. 361, 89- 98. [4] Msiska et al. (2010) Am J Respir Cell Mol Biol. 43, 210–219.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
