The G protein-coupled receptor heterocomplex network database (GPCR-hetnet) is a database designed to store information on GPCR heteroreceptor complexes and their allosteric receptor–receptor interactions. It is an expert-authored and peer-reviewed, curated collection of well-documented GPCR–GPCR interactions that span the gamut from classical GPCR–GPCR interactions to more complex receptor–receptor interactions (GPCR-Receptor Tyrosine Kinase and GPCR-ionotropic receptor/ligand gated ion channel). Although GPCR-hetnet contains interactions among GPCR from several different species, the curators have initially focused on receptor–receptor interactions in humans. Currently (August 2017) GPCR-hetnet contains information on 250 receptors (192 GPCR, 52 RTK, and 6 ionotropic receptors) and >1023 interactions. The GPCR-hetnet provides four searchable datasets: the hetnet, the non-hetnet, the rtknet, and the ionnet. Other supporting datasets include information about receptors that are present in GPCR-hetnet such as literature citations. This chapter describes in a basic protocol how to use, navigate, and browse through the GPCR-hetnet database to identify the clusters in which a receptor protomer of interest is involved, while further applicability are also described and introduced.
Searching the GPCR heterodimer network (GPCR-hetnet) database for information to deduce the receptor–receptor interface and its role in the integration of receptor heterodimer functions / Brito, I.; Narvaez, M.; Savelli, D.; Shumilov, K.; Di Palma, M.; Sartini, S.; Skieterska, K.; Van Craenenbroeck, K.; Valladolid-Acebes, I.; Zaldivar-Oro, R.; Filip, M.; Cuppini, R.; Rivera, A.; Liu, F.; Ambrogini, P.; Perez de la Mora, M.; Fuxe, K.; Borroto-Escuela, D. O.. - ELETTRONICO. - 140:(2018), pp. 283-298. [10.1007/978-1-4939-8576-0_18]