EMBL is operating two beamlines for macromolecular crystallography on PETRA III (DESY, Hamburg). Both beamlines are fully tunable and provide a wide range of beam conditions. High flux X-ray beams with adjustable dimensions between 5 and 200 µm are available in the energy range between 4 and 18 keV. To demonstrate the capability of the beamlines, we will describe and discuss typical experiments including: - Structure solution via S-SAD phasing using 4 keV X-rays on P13. - Structure solution using SAD phasing at 6.5 keV on multiple crystals with linear dimensions < 10 µm. - Structure solution by molecular replacement from data collected using serial helical scans on micro-crystals presented to the beam at room temperature in CrystalDirectTM plates. - Rapid ( < 3 min) data collection using a CRL-collimated X-ray beam with a ‘top-hat’ profile. As a CrystalDirect Harvester system will be installed at EMBL Hamburg in June 2016, we hope to be able to present first results with crystals harvested with this system by the time of the meeting.
P13 and P14, the EMBL Beamlines for Macromolecular Crystallography at PETRA III / Bento, Isabel; Bourenkov, Gleb; Cianci, Michele; Pompidor, Guillaume; Kallio, Johanna; Karpics, Ivars; Polyakova, Anna; Fiedler, Stefan; Schneider, Thomas R.. - In: ACTA CRYSTALLOGRAPHICA. SECTION A, FOUNDATIONS OF CRYSTALLOGRAPHY. - ISSN 0108-7673. - ELETTRONICO. - A72:(2016), p. s194. (Intervento presentato al convegno 30th European Crystallographic Meeting tenutosi a Basel, Switzerland nel 28/08/2016-01/09/2016).
P13 and P14, the EMBL Beamlines for Macromolecular Crystallography at PETRA III
CIANCI, MICHELE;
2016-01-01
Abstract
EMBL is operating two beamlines for macromolecular crystallography on PETRA III (DESY, Hamburg). Both beamlines are fully tunable and provide a wide range of beam conditions. High flux X-ray beams with adjustable dimensions between 5 and 200 µm are available in the energy range between 4 and 18 keV. To demonstrate the capability of the beamlines, we will describe and discuss typical experiments including: - Structure solution via S-SAD phasing using 4 keV X-rays on P13. - Structure solution using SAD phasing at 6.5 keV on multiple crystals with linear dimensions < 10 µm. - Structure solution by molecular replacement from data collected using serial helical scans on micro-crystals presented to the beam at room temperature in CrystalDirectTM plates. - Rapid ( < 3 min) data collection using a CRL-collimated X-ray beam with a ‘top-hat’ profile. As a CrystalDirect Harvester system will be installed at EMBL Hamburg in June 2016, we hope to be able to present first results with crystals harvested with this system by the time of the meeting.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
