Raw cement asbestos (RCA) undergoes a complete solid state transformation when heated at high temperatures (1200 °C). The secondary raw material produced after treatment (HT-CA) is composed of newly-formed crystals in place of the original asbestos fibres present in RCA. Our previous studies (1) showed that HT-CA treatment exerts cytotoxic effects of lower grade compared to RCA. In this study we found that RCA treatment of A549 epithelial cells increase inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) release in the culture media in RCA cells compared to control and HT-CA cells. It is already known that the asbestos-induced inflammatory status may drive lung cells to the carcinogenesis (2), events sustained by iNOS expression and NO production that play a role in cell-mediated immune response in lung cells. Interestingly, HT-CA cell treatment reveals Fe content in cristallized instead of soluble form found in RCA that can be related to the significantly lower toxicity for inertized cement-asbestos. Our findings confirm that HT-CA can be considered a transformed phase exerting direct lower cytotoxic and inflammatory potential compared to RCA on biological systems. High-temperature RCA treatment could represent a safe approach for storing or recycling asbestos materials. Further studies are in progress to exclude any residual carcinogenicity in HT-CA matrix.
Raw and thermally treated cement asbestos exerts different cytotoxic effects and Nitric Oxide production in A549 cells / Pugnaloni, Armanda; Lucarini, Guendalina; Tomasetti, Marco; Strafella, Elisabetta; Alessandro F., Gualtieri. - STAMPA. - 119, N.1 (Supplement):(2014), pp. 158-158. (Intervento presentato al convegno 68° Meeting of the Italian Society of Anatomy and Histology tenutosi a Ancona nel 18-20 september 2014).
Raw and thermally treated cement asbestos exerts different cytotoxic effects and Nitric Oxide production in A549 cells.
PUGNALONI, Armanda
Supervision
;LUCARINI, GuendalinaMembro del Collaboration Group
;TOMASETTI, MarcoMembro del Collaboration Group
;STRAFELLA, ELISABETTAProject Administration
;
2014-01-01
Abstract
Raw cement asbestos (RCA) undergoes a complete solid state transformation when heated at high temperatures (1200 °C). The secondary raw material produced after treatment (HT-CA) is composed of newly-formed crystals in place of the original asbestos fibres present in RCA. Our previous studies (1) showed that HT-CA treatment exerts cytotoxic effects of lower grade compared to RCA. In this study we found that RCA treatment of A549 epithelial cells increase inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) release in the culture media in RCA cells compared to control and HT-CA cells. It is already known that the asbestos-induced inflammatory status may drive lung cells to the carcinogenesis (2), events sustained by iNOS expression and NO production that play a role in cell-mediated immune response in lung cells. Interestingly, HT-CA cell treatment reveals Fe content in cristallized instead of soluble form found in RCA that can be related to the significantly lower toxicity for inertized cement-asbestos. Our findings confirm that HT-CA can be considered a transformed phase exerting direct lower cytotoxic and inflammatory potential compared to RCA on biological systems. High-temperature RCA treatment could represent a safe approach for storing or recycling asbestos materials. Further studies are in progress to exclude any residual carcinogenicity in HT-CA matrix.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
