Analysis of Verdicchio (a white wine produced in Italy) with high performance liquid chromatography-tandem mass spectrometry (HPLC-ESI-MS-MS) showed a concentration of ethyl caffeoate (CfE), a natural phenolic Substance, of 67.3 mu mol L-1; CfE was then purified from wine with preparative reversed phase HPLC and tested on rat hepatic stellate cell cultures (HSC). HSC proliferation induced by iron(III) nitrilotriacetate (FeNTA), a pro-oxidant agent (absorbance = 0.68 +/- 10.03 versus 0.32 +/- 0.02 in controls), was significantly inhibited by CfE starting at a concentration of 10 mu mol L-1 (absorbance = 0.45 +/- 0.01), with a maximal effect at 20 mu mol L-1 (absorbance = 0.32 +/- 0.02). The formation of intracellular hydroperoxides induced by FeNTA was significantly reduced by CfE starting at a concentration of 10 mu mol L-1 and completely prevented by CfE at a concentration of 20 mu mol L-1. The increase in alpha 1 (I) procollagen mRNA synthesis induced by FeNTA was inhibited by CfE starting at a concentration of 10 mu mol L-1 and almost completely at a concentration of 20 mu mol L-1. Thus, Verdicchio wine is a remarkable source (about seven-fold the bioactive concentration) of CfE, an interesting drug to be tested as an antifibrotic agent.

Ethyl caffeoate: Liquid chromatography–tandem mass spectrometric analysis in Verdicchio wine and effects on hepatic stellate cells and intracellular peroxidation / Frega, Natale Giuseppe; Boselli, Emanuele; Bendia, Emanuele; M., Minardi; Benedetti, Antonio. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - 563:(2006), pp. 375-381. [10.1016/j.aca.2005.09.017]

Ethyl caffeoate: Liquid chromatography–tandem mass spectrometric analysis in Verdicchio wine and effects on hepatic stellate cells and intracellular peroxidation

FREGA, Natale Giuseppe;BOSELLI, EMANUELE;BENDIA, EMANUELE;BENEDETTI, Antonio
2006-01-01

Abstract

Analysis of Verdicchio (a white wine produced in Italy) with high performance liquid chromatography-tandem mass spectrometry (HPLC-ESI-MS-MS) showed a concentration of ethyl caffeoate (CfE), a natural phenolic Substance, of 67.3 mu mol L-1; CfE was then purified from wine with preparative reversed phase HPLC and tested on rat hepatic stellate cell cultures (HSC). HSC proliferation induced by iron(III) nitrilotriacetate (FeNTA), a pro-oxidant agent (absorbance = 0.68 +/- 10.03 versus 0.32 +/- 0.02 in controls), was significantly inhibited by CfE starting at a concentration of 10 mu mol L-1 (absorbance = 0.45 +/- 0.01), with a maximal effect at 20 mu mol L-1 (absorbance = 0.32 +/- 0.02). The formation of intracellular hydroperoxides induced by FeNTA was significantly reduced by CfE starting at a concentration of 10 mu mol L-1 and completely prevented by CfE at a concentration of 20 mu mol L-1. The increase in alpha 1 (I) procollagen mRNA synthesis induced by FeNTA was inhibited by CfE starting at a concentration of 10 mu mol L-1 and almost completely at a concentration of 20 mu mol L-1. Thus, Verdicchio wine is a remarkable source (about seven-fold the bioactive concentration) of CfE, an interesting drug to be tested as an antifibrotic agent.
2006
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11566/34390
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