Self-assembling and dynamical properties of deoxyguanosine 5'-monophosphate in isotropic aqueous solutions were studied by 31P NMR spectroscopy and dynamic light scattering (DLS). All solutions had the same c = 4 wt% guanosine concentration, while the added KCl molarity ranged from 0 to 1.5 M. 31P NMR measurements show that potassium ions strongly enhance the stacking process of guanosine tetramers until a saturation is reached at 0.1 M KCl with more than 70% of the molecules aggregated. Polarized light scattering reveals the presence of a fast relaxation mode that arises from the translational dynamics of the self-assembled stacks. The diffusion coefficient of this mode shows a strong dependence on molarity of added salt, which can be described in terms of the coupled mode and counterion condensation theories for polyelectrolyte solutions. Depolarized light scattering reveals the rotational dynamics of the self-assembled stacks which exhibits a pronounced slowing down with increasing the added salt content
Dynamic light scattering and 31P NMR study of the self-assembly of deoxyguanosine 5’-monophosphate: the effect of added salt / Spindler, L.; DREVENSEK OLENIK, I.; Copic, M.; Cerar, J.; Skerjanc, J.; Mariani, Paolo. - In: THE EUROPEAN PHYSICAL JOURNAL. E, SOFT MATTER. - ISSN 1292-8941. - STAMPA. - 13:(2004), pp. 27-33.
Dynamic light scattering and 31P NMR study of the self-assembly of deoxyguanosine 5’-monophosphate: the effect of added salt
MARIANI, Paolo
2004-01-01
Abstract
Self-assembling and dynamical properties of deoxyguanosine 5'-monophosphate in isotropic aqueous solutions were studied by 31P NMR spectroscopy and dynamic light scattering (DLS). All solutions had the same c = 4 wt% guanosine concentration, while the added KCl molarity ranged from 0 to 1.5 M. 31P NMR measurements show that potassium ions strongly enhance the stacking process of guanosine tetramers until a saturation is reached at 0.1 M KCl with more than 70% of the molecules aggregated. Polarized light scattering reveals the presence of a fast relaxation mode that arises from the translational dynamics of the self-assembled stacks. The diffusion coefficient of this mode shows a strong dependence on molarity of added salt, which can be described in terms of the coupled mode and counterion condensation theories for polyelectrolyte solutions. Depolarized light scattering reveals the rotational dynamics of the self-assembled stacks which exhibits a pronounced slowing down with increasing the added salt contentI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
