Overview: The global spread of antibiotic resistance represents a serious threat for public health. Aim: We evaluated the efficacy of the antimicrobial peptide LL-37 as antimicrobial agent against multidrug-resistant Escherichia coli. Results: LL-37 showed good activity against mcr-1 carrying, extended spectrum beta-lactamase- and carbapenemase-producing E. coli (minimum inhibitory concentration, MIC, from 16 to 64mg/l). Checkerboard assays demonstrated synergistic effect of LL-37/colistin combination against all tested strains, further confirmed by time-kill and post antibiotic effect assays. MIC and sub-MIC concentrations of LL-37 were able to reduce biofilm formation. Conclusion: Our preliminary data indicated that LL-37/colistin combination was effective against multidrug resistant E. coli strains and suggested a new possible clinical application.

Synergistic effect of antimicrobial peptide LL-37 and colistin combination against multidrug-resistant Escherichia coli isolates / Morroni, Gianluca; Sante, Laura Di; Simonetti, Oriana; Brescini, Lucia; Kamysz, Wojciech; Kamysz, Elzbieta; Mingoia, Marina; Brenciani, Andrea; Giovanetti, Eleonora; Bagnarelli, Patrizia; Giacometti, Andrea; Cirioni, Oscar. - In: FUTURE MICROBIOLOGY. - ISSN 1746-0913. - (2021). [10.2217/fmb-2020-0204]

Synergistic effect of antimicrobial peptide LL-37 and colistin combination against multidrug-resistant Escherichia coli isolates

Morroni, Gianluca;Simonetti, Oriana
;
Brescini, Lucia;Mingoia, Marina;Brenciani, Andrea;Giovanetti, Eleonora;Bagnarelli, Patrizia;Giacometti, Andrea;Cirioni, Oscar
2021-01-01

Abstract

Overview: The global spread of antibiotic resistance represents a serious threat for public health. Aim: We evaluated the efficacy of the antimicrobial peptide LL-37 as antimicrobial agent against multidrug-resistant Escherichia coli. Results: LL-37 showed good activity against mcr-1 carrying, extended spectrum beta-lactamase- and carbapenemase-producing E. coli (minimum inhibitory concentration, MIC, from 16 to 64mg/l). Checkerboard assays demonstrated synergistic effect of LL-37/colistin combination against all tested strains, further confirmed by time-kill and post antibiotic effect assays. MIC and sub-MIC concentrations of LL-37 were able to reduce biofilm formation. Conclusion: Our preliminary data indicated that LL-37/colistin combination was effective against multidrug resistant E. coli strains and suggested a new possible clinical application.
2021
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11566/288297
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